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Gene for an immunoglobulin-binding protein from a group G streptococcus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fahnestock, Stephen R. Alexander, Peter Nagle, James Filpula, David |
| Copyright Year | 1986 |
| Abstract | The gene (spg) for an immunoglobulin G (IgG)-binding protein from a Streptococcus clinical isolate of Lancefield group G was cloned and expressed in Escherichia coli. The complete nucleotide sequence of the gene and 5'-flanking sequences was determined. The DNA sequence includes an open reading frame which encodes a hypothetical protein of 448 amino acid residues (Mr = 47,595). The 5' end of this open reading frame encodes a sequence resembling a typical secretion signal sequence, and the remainder of the encoded protein has features reminiscent of staphylococcal protein A and of streptococcal M6 protein, including repeated sequences and a similar C-terminal structure. Aside from this C-terminal structure, the encoded protein has little direct amino acid sequence homology to either protein A or M6 protein. In E. coli, the cloned gene directs the synthesis of a protein which binds to immunoglobulins, including rabbit immunoglobulin, goat IgG, and human IgG3(lambda). Its binding properties are similar to those of the protein G described by Björck and Kronvall (L. Björck and G. Kronvall, J. Immunol. 133:969-974, 1984), a type III Fc receptor from a group G streptococcus. |
| Starting Page | 299 |
| Ending Page | 305 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jb.asm.org/content/167/3/870.full.pdf |
| PubMed reference number | 3745123v1 |
| Volume Number | 167 |
| Issue Number | 3 |
| Journal | Journal of bacteriology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Sequence Amino Acids BSG gene Base Sequence Carboxy-Terminal Amino Acid Clone Cells Fc Receptor Homologous Gene Immunoglobulin G Immunoglobulins Nucleotides Open Reading Frames Reading Frames (Nucleotide Sequence) Sequence Homology Sequence Homology, Amino Acid Signal Peptides Staphylococcal Protein A Streptococcal Infections Streptococcus agalactiae |
| Content Type | Text |
| Resource Type | Article |
