NDLI: Quantitative determination of lappaconitine in plasma by liquid chromatography-tandem mass spectrometry and its application in the pharmacokinetic study in rabbits

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Quantitative determination of lappaconitine in plasma by liquid chromatography-tandem mass spectrometry and its application in the pharmacokinetic study in rabbits

Content Provider	Semantic Scholar
Author	Wang, Yingzi Han, Chunchao He, Kuibang Feng, Ailing
Copyright Year	2013
Abstract	A high sensitive and rapid method was developed for the analysis of lappaconitine in rabbit plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Blood was taken from the ear vein after transdermal administration of lappaconitine gel on the back of male rabbits, followed by liquid-liquid extraction with n-hexane from plasma, the analyte was separated in an Inertsil ODS-3 column (2.1×50mm, 0.5μm) through gradient elution with acetonitrile-water-formic acid at a flow rate of 0.4 mL/min. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 585.5 → m/z 535.5 and m/z 531.3 → m/z 489.4, for the quantification of lappaconitine and the internal standard (IS) ketonconazole, respectively. The method was linear over the concentration range of 13.125-1050.0ng/mL and the lower limit of quantification was 13.125 ng/mL. The intra- and inter-day precisions were less than 7.51%, and accuracy ranged from 92.3 to 102%. Under the transdermal concentrations of 13.125n g/mL, 65.625 ng/mL and 525.00 ng/mL, the absolute recoveries of lappaconitine were 77.8, 84.4 and 79.0%, respectively. The concentration versus time curve in 24 h was fitted using the bimodal model of DAS ver1.0 software and the results showed that the elimination process of lappaconitine in the body of rabbit was similar to the one-compartment model. Key words: Lappaconitine, LC-MS/MS, plasma drug concentration, pharmacokinetics.
Starting Page	650
Ending Page	657
Page Count	8
File Format	PDF HTM / HTML
DOI	10.5897/AJPP12.1301
Volume Number	7
Alternate Webpage(s)	https://academicjournals.org/journal/AJPP/article-full-text-pdf/6C35C9B32888
Alternate Webpage(s)	https://doi.org/10.5897/AJPP12.1301
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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