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Hydrazide-functionalized poly(2-hydroxyethyl methacrylate) microspheres for immobilization of horseradish peroxidase.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Horák, Daniel Karpisek, Michal Turková, Jaroslava Benes, Michal |
| Copyright Year | 1999 |
| Abstract | Nonporous cross-linked poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (poly(HEMA-co-EDMA)) microspheres were prepared by dispersion polymerization of HEMA and EDMA. The polymerization was performed in toluene/2-methylpropan-1-ol in the presence of cellulose acetate butyrate as a steric stabilizer and dibenzoyl peroxide initiator. The particle size may be increased by decreasing the toluene/2-methylpropan-1-ol ratio and by increasing polymerization temperature. Adipohydrazide was attached to the microspheres activated with 2,4,6-trichloro-1,3,5-triazine. After periodate oxidation of its carbohydrate moieties, horseradish peroxidase was coupled to the hydrazide-functionalized poly(HEMA-co-EDMA) microparticles up to 7.3 microgram of enzyme/g of carrier without a significant loss of its activity. Immobilized peroxidase was found to be stable, retaining more than 97% of its initial activity when stored for 23 days after the preparation. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/bp990006w |
| PubMed reference number | 10194396 |
| Journal | Medline |
| Volume Number | 15 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://lib3.dss.go.th/fulltext/Journal/Biotechnology%20Progress/Biotechnology%20Progress/1999/no.2/1999v15n2p.208-215.pdf |
| Journal | Biotechnology progress |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
