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Platelet function alterations in dengue are associated with plasma leakage.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Michels, Meta Alisjahbana, Bachti Groot, Philip Ph. G. De Indrati, Amalia Rahmaniar Fijnheer, Rob Puspita, Merry Dewi, Intan Mauli Warma Wijer, Lisa Van De Boer, Emelie M. De Roest, Mark R. Ven, Andre J. Van Der Mast, Quirijn De |
| Copyright Year | 2014 |
| Abstract | Severe dengue is characterised by thrombocytopenia, plasma leakage and bleeding. Platelets are important for preservation of endothelial integrity. We hypothesised that platelet activation with secondary platelet dysfunction contribute to plasma leakage. In adult Indonesian patients with acute dengue, we measured platelet activation status and the response to the platelet agonist TRAP using flow cytometer-based assays. Patients were monitored daily for plasma leakage by ultrasonography. Acute dengue was associated with platelet activation with an increased expression of the activated fibrinogen receptor (αIIbβ3), the lysosomal marker CD63 and the alpha-granule marker CD62P (P-selectin). Upon maximal platelet activation by TRAP, platelet function defects were observed with a significantly reduced maximal activated αIIbβ3 and CD63 expression and reduced platelet-monocyte and platelet-neutrophil complexes. Patients in the lowest tertile of activated αIIbβ3 and CD63 expression had an odds ratio for plasma leakage of 5.2 (95% confidence interval [CI] 1.3-22.7) and 3.9 (95% CI 1.1-13.7), respectively, compared to the highest tertile. Platelet-derived serotonin has previously been related to plasma leakage and we found increased intra-platelet serotonin concentrations in our patients. In conclusion, platelet activation with platelet function alterations can be found in patients with acute dengue and this may contribute to dengue-associated plasma leakage. |
| Starting Page | 143 |
| Ending Page | 150 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://th.schattauer.de/contents/archive/issue/1895/manuscript/21012/download.html |
| PubMed reference number | 24695691v1 |
| Alternate Webpage(s) | https://doi.org/10.1160/TH14-01-0056 |
| DOI | 10.1160/th14-01-0056 |
| Journal | Thrombosis and haemostasis |
| Volume Number | 112 |
| Issue Number | 2 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Antigens, CD63 Biologic Preservation Blood Platelets Confidence Intervals Dengue Fever Extravasation Hemorrhage Leukemia, Myelocytic, Acute P-Selectin Patients Platelet Activation Platelet Count measurement Receptors, Fibrinogen Selectins Serotonin Thrombocytopenia automated flow cytometers neutrophil |
| Content Type | Text |
| Resource Type | Article |
