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Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Xueqian Dong, Yanpeng Zhang, Jin-You Zhang, Aixiang Wang, Lei Feng, Lu |
| Copyright Year | 2009 |
| Abstract | Two alkyl alcohol dehydrogenase (ADH) genes from the long-chain alkane-degrading strain Geobacillus thermodenitrificans NG80-2 were characterized in vitro. ADH1 and ADH2 were prepared heterologously in Escherichia coli as a homooctameric and a homodimeric protein, respectively. Both ADHs can oxidize a broad range of alkyl alcohols up to at least C(30), as well as 1,3-propanediol and acetaldehyde. ADH1 also oxidizes glycerol, and ADH2 oxidizes isopropyl alcohol, isoamylol, acetone, octanal and decanal. The best substrate is ethanol for ADH1 and 1-octanol for ADH2. For both ADHs, the optimum assay condition is at 60 degrees C and pH 8.0, and both NAD and NADP can be used as the cofactor. Sequence analysis reveals that ADH1 and ADH2 belong to the Fe-containing/activated long-chain ADHs. However, the two enzymes contain neither Fe nor other metals, and Fe is not required for the activity, suggesting a new type of ADH. The ADHs characterized here are potentially useful in crude oil bioremediation and other bioconversion processes. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/mic.0.027201-0 |
| PubMed reference number | 19383697 |
| Journal | Medline |
| Volume Number | 155 |
| Part | 6 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/155/6/2078.pdf?accname=guest&checksum=5FD76BC311671F406EDA33E9D14CB8AD&expires=1539475778&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/micro/155/6/2078.pdf?accname=guest&checksum=2BF50BEDB316046F4A607112D62D9B0C&expires=1545993728&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/155/6/2078.pdf?accname=guest&checksum=FC88873C1D17831D8DC74F6F7FDC9D4A&expires=1542805937&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/micro/155/6/2078.pdf?accname=guest&checksum=93168CDB64ACFD7AAD83A133115FE7FA&expires=1564977501&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/micro/155/6/2078.pdf?accname=guest&checksum=9F8087835A3543692456EDDABF25AF72&expires=1549312724&id=id |
| Journal | Microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
