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Determination of Methylene Blue Drug Residues in Import and Export Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry *
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yin, Ju-Yi Chen, Jie Tang, Haiqing Mo, Yanxia Gu, Xiaojun Chen, Meizhen Wu, Weier He, Wei-Min |
| Copyright Year | 2013 |
| Abstract | In this study, a HPLC-ESI(+)-MS/MS methodology is used for the high sensitive determination of drug residue of methylene blue (MB) in import and export aquatic products. The sample was extracted by the acetonitrile and operated with a neutral alumina column solid-phase extraction column. After elution, it was evaporated to dryness. The residue was dissolved by constant volume liquid and was filtered through 0.45-μm membrane. The sample solution was for determination of liquid chromatography-tandem mass spectrometry. The liquid chromatography tandem mass spectrometry was operated in the positive ion mode, using multiple reaction monitoring (MRM) for qualitative and quantitative analysis according to the calibration curve and the external standard method. Samples were detected with a ESI positive mode detector at MRM: m/z 285.1/269.1 and m/z 285.1/241.0. In the line of quality assurance, the stated LOQ of methylene blue residues in the negative sample was at the level of 0.1 μg/kg. The response for methylene blue was linear in the range of 0.5 50.0 μg/L, and the correlation coefficient is excellent 0.999. The average recovery range of this method was 87.6% 111.2%, and the range of RSD was 4.0% 10.2%. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.hanspub.org/journal/PaperDownload.aspx?DOI=10.12677/HJFNS.2013.24014 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
