Label-free triple-helix aptamer as sensing platform for "signal-on" fluorescent detection of thrombin.

Content Provider	Semantic Scholar
Author	Wang, Quanbo Lei, Jianping Ju, Huangxian
Copyright Year	2015
Abstract	The design of a label-free aptamer for separation of recognition sequence from signal reporter is significant to ensure the high-efficiency affinity between aptamer and target. This work develops a label-free triple-helix aptamer (THA) as sensing platform for "signal-on" fluorescent detection of thrombin. THA was composed of aptamer sequence and help DNA 1 (H1), which contained the complementary sequence of hexachloro-fluorescein (HEX) labeled help DNA 2 (H2). The specific recognition event between aptamer and thrombin triggered the dismission of THA to release H1. The released H1 then reacted with the signal probe of H2/graphene oxide (GO) nanocomposite to form H1-H2 duplex, leading to the fluorescence recovery of H2 due to the detachment of H1-H2 duplex from the surface of GO. With employment of THA as a signal transducer and GO as a "superquencher", this method shows a sensitive response to thrombin with a wide concentration range from 5 to 1200 nM. The limit of detection is 1.8 nM (S/N=3) with excellent selectivity. Considering the universality of THA, the proposed aptasensor would provide a platform for homogeneous fluorescent detection of a wide range of analytes.
File Format	PDF HTM / HTML
DOI	10.1016/j.talanta.2014.09.031
PubMed reference number	25476322
Journal	Medline
Volume Number	132
Alternate Webpage(s)	http://sklac.nju.edu.cn/hxju/lunwenlunzhu/paper2015/485%20Talanta%20Xu%20Nan.pdf
Journal	Talanta
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article