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Metabolism of 2,4-dichlorophenoxyacetic Acid in soybean root callus and differentiated soybean root cultures as a function of concentration and tissue age.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Davidonis, Gayle H. Hamilton, Robert H. Mumma, Ralph O. |
| Copyright Year | 1978 |
| Abstract | The metabolism of [1-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) in soybean (Glycine max [L.] Merrill var. Amsoy) root callus and in differentiated soybean root cultures was investigated as a function of pesticide concentration and age of tissue. The chronological age of the tissue was found to be correlated with the mitotic index which reached a peak at 2 weeks and then declined. The metabolism of 2,4-D changed with age of the root callus tissue. The amount of free 2,4-D found in 3-week-old root callus tissue rapidly increased as the concentration of 2,4-D in the medium was increased from 10(-6) to 10(-5) molar, whereas the low level of aqueous (glycosides) and ether soluble metabolites (2,4-D amino acid conjugates) increased slowly. With 9-week-old root callus tissue, the amount of free 2,4-D remained at a relatively low, constant level (saturation level) as the concentration of 2,4-D in the medium increased. Under these conditions the aqueous metabolites increased only slightly but the ether fraction (2,4-D amino acid conjugates) rapidly increased. Thus, the older root callus tissue appeared to regulate the level of free 2,4-D at about 4 nanomoles per gram by converting any excess 2,4-D into amino acid conjugates.In 3-week-old, differentiated root cultures the metabolism of 2,4-D closely paralleled the metabolism found in the older 9-week-old callus tissue. The saturation level of free 2,4-D found in this tissue was only about 1 to 2 nanomoles per gram. |
| File Format | PDF HTM / HTML |
| DOI | 10.1104/pp.62.1.80 |
| PubMed reference number | 16660474 |
| Journal | Medline |
| Volume Number | 62 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/plantphysiol/62/1/80.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1104/pp.62.1.80 |
| Journal | Plant physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
