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Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hasan, Salah D. M. Gambale, Walderez Zollner, Ricardo De Lima Santana, Maria Helena Andrade |
| Copyright Year | 2003 |
| Abstract | Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays. |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/978-1-4612-0057-4_33 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/978-1-4612-0057-4_33 |
| Alternate Webpage(s) | https://page-one.live.cf.public.springer.com/pdf/preview/10.1007/978-1-4612-0057-4_33 |
| PubMed reference number | 12721463 |
| Alternate Webpage(s) | https://doi.org/10.1007/978-1-4612-0057-4_33 |
| Journal | Medline |
| Volume Number | 105-108 |
| Journal | Applied biochemistry and biotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
