NDLI: fl uorescent resonant energy transfer and the antenna e ff ect in DNA structures with multiple fl uorescent dyes †

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fl uorescent resonant energy transfer and the antenna e ff ect in DNA structures with multiple fl uorescent dyes †

Content Provider	Semantic Scholar
Author	Oh, Taeseok Kim, Sejung Choi, Jae-Young Changb, Haeun Heller, Michael J.
Copyright Year	2017
Abstract	This study examines the use of surfactants andmetal cations for the enhancement of long range fluorescent resonant energy transfer (FRET) and the antenna effect in double-stranded (ds) DNA structures formed by hybridization of 21mer oligonucleotides with three fluorescent TAMRA donor dyes and complementary 21mer oligonucleotides with one fluorescent Texas Red acceptor dye. In FRET ds-DNA structures, hydrophobic interactions between the fluorescent dyes in close proximity produces dimerization and quenching which reduces fluorescent emissions. For the neutral surfactant Triton X-100, dimerization and emission quenching in the FRET ds-DNA structures remain unaffected. The cationic surfactant CTAB (>100 mM), which neutralizes the negatively charged ds-DNA backbone reduces TAMRA dye dimerization and emission quenching, and improves the Texas Red quantum yield, FRET efficiency and the antenna effect. While the negatively charged SDS surfactant does not reduce dimerization and emission quenching, addition of sodium cations (Na) and magnesium cations (Mg) lead to a significant reduction in dimerization and emission quenching, and produce higher FRET efficiency and enhanced antenna effect. This study provides a viable strategy for using combinations of surfactants and cations to reduce fluorescent dye and other quenching mechanisms and improve the overall long distance FRET efficiency and the antenna effect in ds-DNA structures.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://pubs.rsc.org/en/Content/ArticlePDF/2017/RA/C7RA01470H
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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