NDLI: Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

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Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Content Provider	Semantic Scholar
Author	Salehi, Hamideh Middendorp, Elodie Panayotov, Ivan Dutilleul, Pierre-Yves Collard Végh, Attila-Gergely Ramakrishnan, Sathish Kumar Gergely, Csilla Cuisinier, Frédéric
Copyright Year	2013
Abstract	Abstract. Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.
File Format	PDF HTM / HTML
PubMed reference number	23698321
Journal	Medline
Volume Number	18
Alternate Webpage(s)	https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.18.5.056010
Alternate Webpage(s)	http://real.mtak.hu/7478/7/SalehiHJBiomedOptCorr.pdf
Alternate Webpage(s)	http://biomedicaloptics.spiedigitallibrary.org/pdfaccess.ashx?url=/data/journals/biomedo/926860/jbo_18_5_059801.pdf
Alternate Webpage(s)	https://www.spiedigitallibrary.org/journalArticle/Download?fullDOI=10.1117/1.JBO.18.5.059801
Alternate Webpage(s)	http://real.mtak.hu/7478/1/SalehiHJBiomedOpt.pdf
Alternate Webpage(s)	https://doi.org/10.1117/1.JBO.18.5.056010
Journal	Journal of biomedical optics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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