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Fluorescence imaging with one nanometer accuracy: application to molecular motors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yildiz, Ahmet Selvin, Paul R. |
| Copyright Year | 2005 |
| Abstract | We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within approximately 1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion. |
| Starting Page | 114 |
| Ending Page | 120 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=42AE516CCE014915AFC160C1C38BFEF3?doi=10.1.1.391.7084&rep=rep1&type=pdf |
| Alternate Webpage(s) | http://www.s.kanazawa-u.ac.jp/phys/biophys/References/Myosin%20V/Fiona-2005.pdf |
| Alternate Webpage(s) | http://research.physics.berkeley.edu/yildiz/papers/fiona.pdf |
| Alternate Webpage(s) | http://research.physics.berkeley.edu/yildiz/papers/ACR%20review.pdf |
| Alternate Webpage(s) | http://people.physics.illinois.edu/Selvin/PRS/PSCV/PTP/2005/ar040136s_.pdf |
| PubMed reference number | 16028892v1 |
| Volume Number | 38 |
| Issue Number | 7 |
| Journal | Accounts of chemical research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Dyes Fiona Fluorescence Imaging Magnetic Resonance Imaging Myosin Light Chain Kinase Nanometer Normal Statistical Distribution |
| Content Type | Text |
| Resource Type | Article |
