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Ferulago angulata activates intrinsic pathway of apoptosis in MCF-7 cells associated with G1 cell cycle arrest via involvement of p21/p27
| Content Provider | Semantic Scholar |
|---|---|
| Author | Karimian, H. S. M. Moghadamtousi, Soheil Zorofchian Fadaeinasab, Mehran Golbabapour, Shahram Razavi, Mahboubeh Hajrezaie, Maryam Arya, Aditya Abdulla, Mahmood Ameen Mohan, Syam Ali, Hapipah Binti Mohd Noordin, Mohamad Ibrahim |
| Copyright Year | 2014 |
| Abstract | Ferulago angulata is a medicinal plant that is traditionally known for its anti-inflammatory and antiulcer properties. The present study was aimed to evaluate its anticancer activity and the possible mechanism of action using MCF-7 as an in vitro model. F. angulata leaf extracts were prepared using solvents in the order of increasing polarity. As determined by MTT assay, F. angulata leaves hexane extract (FALHE) revealed the strongest cytotoxicity against MCF-7 cells with the half maximal inhibitory concentration (IC50) value of 5.3 ± 0.82 μg/mL. The acute toxicity study of FALHE provided evidence of the safety of the plant extract. Microscopic and flow cytometric analysis using annexin-V probe showed an induction of apoptosis in MCF-7 by FALHE. Treatment of MCF-7 cells with FALHE encouraged the intrinsic pathway of apoptosis, with cell death transducing signals that reduced the mitochondrial membrane potential with cytochrome c release from mitochondria to cytosol. The released cytochrome c triggered the activation of caspase-9. Meanwhile, the overexpression of caspase-8 suggested the involvement of an extrinsic pathway in the induced apoptosis at the late stage of treatment. Moreover, flow cytometric analysis showed that FALHE treatment significantly arrested MCF-7 cells in the G1 phase, which was associated with upregulation of p21 and p27 assessed by quantitative polymerase chain reaction. Immunofluorescence and the quantitative polymerase chain reaction analysis of MCF-7 cells after treatment with FALHE revealed an upregulation of Bax and a downregulation of Bcl-2 proteins. These findings proposed that FALHE suppressed the proliferation of MCF-7 cells via cell cycle arrest and the induction of apoptosis through intrinsic pathway. |
| Starting Page | 1481 |
| Ending Page | 1497 |
| Page Count | 17 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.dovepress.com/getfile.php?fileID=21694 |
| Alternate Webpage(s) | http://repository.um.edu.my/40912/1/f_DDDT-68818-ferulago-angulata-activates-intrinsic-pathway-of-apoptosis-i_092214_21694.pdf |
| PubMed reference number | 25278746v1 |
| Alternate Webpage(s) | https://doi.org/10.2147/DDDT.S68818 |
| DOI | 10.2147/dddt.s68818 |
| Journal | Drug design, development and therapy |
| Volume Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Abbreviations Adverse reaction to drug Annexins Biochemical Pathway Cardiac Arrest Cell Cycle Arrest Cell Death Cell Proliferation Cellular Phone Cytoplasmic matrix Down-Regulation FR 139317 Ferulago angulata Fluorescein Fluorescein-5-isothiocyanate Inhibitory Concentration 50 MCF-7 Cells Manuscripts Membrane Potential, Mitochondrial Membrane Potentials Mitochondria Mitochondrial Membranes Mitosis Neoplasms Plant Extracts Plant Leaves Plants, Medicinal Polymerase Chain Reaction Positive Regulation of Apoptosis Sodium Symphytum uplandicum leaf extract Unit per Liter Up-Regulation (Physiology) adenotonsillectomy caspase caspase-8 caspase-9 monooxyethylene trimethylolpropane tristearate n-hexane polarity |
| Content Type | Text |
| Resource Type | Article |
