NDLI: Alternative reading frame selection mediated by a tRNA-like domain of an internal ribosome entry site.

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Alternative reading frame selection mediated by a tRNA-like domain of an internal ribosome entry site.

Content Provider	Semantic Scholar
Author	Ren, Qian Wang, Qing Song Firth, Andrew E. Chan, Mandy M. Y. Gouw, Joost W. Guarna, M. Marta Foster, Leonard J. Atkins, John F. Jan, Eric
Copyright Year	2012
Abstract	The dicistrovirus intergenic region internal ribosome entry site (IRES) utilizes a unique mechanism, involving P-site tRNA mimicry, to directly assemble 80S ribosomes and initiate translation at a specific non-AUG codon in the ribosomal A site. A subgroup of dicistrovirus genomes contains an additional stem-loop 5'-adjacent to the IRES and a short open reading frame (ORFx) that overlaps the viral structural polyprotein ORF (ORF2) in the +1 reading frame. Using mass spectrometry and extensive mutagenesis, we show that, besides directing ORF2 translation, the Israeli acute paralysis dicistrovirus IRES also directs ORFx translation. The latter is mediated by a UG base pair adjacent to the P-site tRNA-mimicking domain. An ORFx peptide was detected in virus-infected honey bees by multiple reaction monitoring mass spectrometry. Finally, the 5' stem-loop increases IRES activity and may couple translation of the two major ORFs of the virus. This study reveals a novel viral strategy in which a tRNA-like IRES directs precise, initiator Met-tRNA-independent translation of two overlapping ORFs.
File Format	PDF HTM / HTML
PubMed reference number	22247292
Journal	Medline
Volume Number	109
Issue Number	11
Alternate Webpage(s)	http://www.pnas.org/content/109/11/E630.full.pdf?with-ds=yes
Alternate Webpage(s)	http://www.pnas.org/content/109/11/E630/1.full.pdf
Alternate Webpage(s)	http://www.pnas.org/content/109/11/E630.full.pdf
Alternate Webpage(s)	https://doi.org/10.1073/pnas.1111303109
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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