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Cystamine prevents ischemia-reperfusion injury by inhibiting polyamination of RhoA.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shin, Dong-Myung Kang, Jinmo Ha, Jongwon Kang, H. Park, Sang-Chul Kim, In-Gyu Kim, S. J. |
| Copyright Year | 2008 |
| Abstract | Transglutaminase2 (TGase2) activates Rho-associated kinase (ROCK), an important mediator of ischemia-reperfusion (IR) injury, through polyamination of RhoA. Cystamine, an oxidized dimer of cysteamine inhibits the transamidation activity of TGase2. We examined whether addition of cystamine to an organ preservation solution protects rat cardiomyocyte cells (H9C2) from cell death in IR injury. H9C2 cells were stored under hypoxic conditions at 4 degrees C in laboratory-made preservation solution (SNU) or SNU solution supplemented with cystamine (SNU-C1), and cell preservation in the two solutions was compared by measuring the release of lactate dehydrogenase. The cells were preserved more effectively in SNU-C1 than in SNU solution. Cystamine inhibited the intracellular activity of TGase2 which increased during cold storage or reoxygenation. The inhibition of TGase2 by cystamine reduced the polyamination of RhoA, the interaction between RhoA and ROCK2, and F-actin formation. Cystamine also prevented the activation of caspases during cold storage. These results suggest that addition of cystamine to the organ preservation solution significantly enhances cardiomyocytes preservation apparently by inhibiting TGase2-mediated RhoA-ROCK pathway and that TGase2 may play an important role in IR injury by regulating ROCK. |
| Starting Page | 143 |
| Ending Page | 146 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://s-space.snu.ac.kr/bitstream/10371/67511/1/Shin-2008-Cystamine%20prevents%20i.pdf |
| PubMed reference number | 17999914v1 |
| Volume Number | 365 |
| Issue Number | 3 |
| Journal | Biochemical and biophysical research communications |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biologic Preservation Cell Death Cerebral Hypoxia-Ischemia Cessation of life Cryopreservation Cystamine Cysteamine F-Actin Hypoxia Hypoxia-Ischemia, Brain Inhibition Lactate Dehydrogenase Lactic acid Mediator brand of benfluorex hydrochloride Myocytes, Cardiac Organ Preservation Reperfusion Injury Reperfusion Therapy Solutions caspase rhoA GTP-Binding Protein |
| Content Type | Text |
| Resource Type | Article |
