Human foamy virus genome possesses an internal, Bel-1-dependent and functional promoter.

Content Provider	Semantic Scholar
Author	Löchelt, Martin Muranyi, Walter Flügel, Rolf M.
Copyright Year	1993
Abstract	The human foamy or spumaretrovirus (HSRV) is a complex retrovirus that encodes the three retroviral genes gag, pol, and env and, in addition, at least three bel genes. The HSRV Bel-1 protein was identified as a transcriptional trans-activator. HSRV transcription starts in the 5' long terminal repeat at a defined guanine residue. We report here that a second efficiently utilized start site of transcription is contained within a HSRV env DNA sequence upstream of the bel genes. Bel-specific transcripts that initiate at the internal transcriptional start site at nt 9196 were identified in HSRV-infected cells by primer extension and S1 nuclease analysis, and the intragenic promoter was shown to be constitutively activated by Bel-1 in the HSRV provirus. In transient expression assays with indicator gene constructs, expression by the HSRV intragenic promoter/enhancer is Bel-1 dependent. The data provide evidence for an intragenic start site of transcription in the genome of a complex, exogenous human retrovirus and are discussed in terms of a model for regulating spumaretroviral gene expression.
Starting Page	293
Ending Page	300
Page Count	8
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/90/15/7317.full.pdf
PubMed reference number	8394017v1
Volume Number	90
Issue Number	15
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Contain (action) Enhancer of transcription Gene Expression Guanine Long Terminal Repeat Primer Extension Proviruses Retroviridae Spumavirus Terminal Repeat Sequences Trans-Activators Transcript Transcription, Genetic nuclease
Content Type	Text
Resource Type	Article