Loading...
Please wait, while we are loading the content...
Similar Documents
Improved method for determining antibiotic susceptibility of Flavobacterium columnare isolates by broth microdilution.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Darwish, Ahmed M. Farmer, Bradley D. Hawke, John Phillip |
| Copyright Year | 2008 |
| Abstract | A simple and reproducible microdilution method was developed to test the susceptibility of the bacterium Flavobacterium columnare to antibiotics in vitro. The testing was conducted at 28 degrees C for 44-48 h at two dilutions of Mueller-Hinton broth (DMHB) using a standardized inoculum, a reference isolate of Escherichia coli ATCC25922 as a quality control organism, positive and negative control wells, and standardized custom-made microtiter plates. The E. coli ATCC25922 and F. columnare ATCC23463 (the species type strain) had significantly better growth in DMHB at 1:5 (4 g/L) than at 1:7 (3 g/L). The E. coli ATCC25922 was found to be acceptable as a reference isolate and produced minimum inhibitory concentration (MIC) values similar to those in the range published by the Clinical and Laboratory Standards Institute derived using standard Mueller-Hinton broth. The new method was used to determine the MIC of 23 F. columnare isolates (representing the three genotypes of the species) to enrofloxacin, ampicillin, oxytetracycline, erythromycin, florfenicol, flumequine, ormetoprim/sulfadimethoxine, and oxolinic acid. |
| Starting Page | 5896 |
| Ending Page | 5900 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://naldc.nal.usda.gov/download/55370/PDF |
| PubMed reference number | 19306607v1 |
| Alternate Webpage(s) | https://doi.org/10.1577/H07-047.1 |
| DOI | 10.1577/H07-047.1 |
| Journal | Journal of aquatic animal health |
| Volume Number | 20 |
| Issue Number | 4 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Ampicillin Campylopus flavicomus Erythromycin Escherichia coli Infections Flavobacterium columnare Geiger-Mueller Counters Genotype Oxolinic Acid Oxytetracycline Scientific Publication Water Wells enrofloxacin florfenicol flumequine ormetoprim / Sulfadimethoxine standards characteristics |
| Content Type | Text |
| Resource Type | Article |
