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Cytokinin a⁄nity puri¢cation and identi¢cation of a tobacco BY-2 adenosine kinase
| Content Provider | Semantic Scholar |
|---|---|
| Author | Laukensa, Kris Strnadb, Miroslav Onckelena, Harry Van Wittersa, Erwin |
| Copyright Year | 2002 |
| Abstract | Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin a⁄nity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identi¢ed by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adeninebut not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the ¢rst time a plant adenosine kinase a⁄nity-puri¢ed to homogeneity that was identi¢ed by primary structure analysis. ' 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://doc.anet.be/docman/docman.phtml?file=.irua.f01560.5195.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
