NDLI: SUSP1 antagonizes formation of highly SUMO2/3-conjugated species

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SUSP1 antagonizes formation of highly SUMO2/3-conjugated species

Content Provider	Semantic Scholar
Author	Mukhopadhyay, Debaditya Ayaydin, Ferhan Kolli, Nagamalleswari Tan, Shyh-Han Anan, Tadashi Kametaka, Ai Azuma, Yoshiaki Wilkinson, Keith D. Dasso, Mary
Copyright Year	2006
Abstract	Small ubiquitin-related modifier (SUMO) processing and deconjugation are mediated by sentrin-specific proteases/ubiquitin-like proteases (SENP/Ulps). We show that SUMO-specific protease 1 (SUSP1), a mammalian SENP/Ulp, localizes within the nucleoplasm. SUSP1 depletion within cell lines expressing enhanced green fluorescent protein (EGFP) fusions to individual SUMO paralogues caused redistribution of EGFP-SUMO2 and -SUMO3, particularly into promyelocytic leukemia (PML) bodies. Further analysis suggested that this change resulted primarily from a deficit of SUMO2/3-deconjugation activity. Under these circumstances, PML bodies became enlarged and increased in number. We did not observe a comparable redistribution of EGFP-SUMO1. We have investigated the specificity of SUSP1 using vinyl sulfone inhibitors and model substrates. We found that SUSP1 has a strong paralogue bias toward SUMO2/3 and that it acts preferentially on substrates containing three or more SUMO2/3 moieties. Together, our findings argue that SUSP1 may play a specialized role in dismantling highly conjugated SUMO2 and -3 species that is critical for PML body maintenance.
Starting Page	939
Ending Page	949
Page Count	11
File Format	PDF HTM / HTML
PubMed reference number	17000875
Volume Number	174
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/174/7/939.full.pdf
Alternate Webpage(s)	https://kuscholarworks.ku.edu/bitstream/handle/1808/15208/AzumaY_2006.pdf;jsessionid=B33F70BF9CA85C9ED56D3019BC6C169D?sequence=1
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/174/7/939.full.pdf?with-ds=yes
Language	English
Access Restriction	Open
Subject Keyword	Chemical Modifier Cultured Cell Line Endopeptidases Enlargement procedure Green Fluorescent Proteins Immunostimulating conjugate (antigen) Leukemia, B-Cell Mammals Nucleoplasm Polyvinyl Chloride SENP6 gene SENP6 wt Allele SUMO-1 Protein Sulfones Vigabatrin enhanced green fluorescent protein leukemia
Content Type	Text
Resource Type	Article

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