Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

Content Provider	Semantic Scholar
Author	Moffitt, Jeffrey R. Bambah-Mukku, Dhananjay Eichhorn, Stephen W. Vaughn, Eric Martin Shekhar, K. Pérez, Julio David Buldain Rubinstein, Nimrod D. Hao, Junjie Regev, Aviv Dulac, Catherine Zhuang, Xiaowei
Copyright Year	2018
Abstract	The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei—including the molecular identity, spatial organization, and function of distinct cell types—is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled ~1 million cells, identified ~70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.
File Format	PDF HTM / HTML
DOI	10.1126/science.aau5324
PubMed reference number	30385464
Journal	Medline
Volume Number	362
Alternate Webpage(s)	http://genetics.wustl.edu/bio5488/files/2019/02/2019_3D-genome_Reading3_Moffitt2018.pdf
Journal	Science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article