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Resfriamento e criopreservação de sêmen de peixe teleósteo Piabanha (Brycon insignis)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Amaral, Thiciana Barbosa Do |
| Copyright Year | 2014 |
| Abstract | AMARAL, Thiciana Barbosa do. Cooling storage and cryopreservation of teleost fish piabanha Brycon insignis sperm. 2009. 51 p. Dissertation (Master in Veterinary Science) – Universidade Federal de Lavras, Lavras. Piabanha (Brycon insignis) is a native species of Paraiba do Sul River Basin, and is presently set as vulnerable. The survival of this specie is threatened by pollution of the river, caused by domestic, industrial and agricultural discharge, and by the introduction of the feral jaw characin (Salminus maxillosus). Piabanha is high-priced market and very appreciated for sport fishing. The aim of this study was to develop a protocol for sperm preservation of piabanha, both by cooling at 4oC -6oC and by freezing. To develop a cooling protocol for sperm, simple extenders (NaCl and glucose) combined or not with gentamycin, and complex extenders (BTS and Androstar) in different osmolalities were tested. Motility, quality score and motility duration was evaluated after 0, 2 and 4 days after cooling and sperm motility was activated with NaCl 0.29% and NaHCO3 1%. To develop a freezing protocol for sperm, eight freezing media composed of four extenders (NaCl, glucose, BTS and M III) and two cryoprotectants (dimethylsulphoxide-DMSO and methylglycol), two thawing temperatures (30 and 60°C) and two activation media (NaCl 0.29% and NaHCO3 1%) were tested. Sperm was frozen in 0.5-mL straws in nitrogen vapor at -170°C and stored in liquid nitrogen. Motility, quality score and motility duration was evaluated after thawing. During the cooling experiments, all diluted and control samples possessed high quality on day 0 of storage. On day 2, the addition of gentamycin was beneficial when added to NaCl solution but not glucose solution. Only samples diluted in BTS and in Androstar yielded motility above 50%, quality score above 1.9 and motility duration above 50 s. When this part of experiment was repeated, highest motility (60-63%) and duration (111-117 s) were observed on samples diluted in Androstar at 322 and 362 mOsm, and in control samples. Sperm quality in all samples was higher when NaHCO3 1% was used as activation medium. On day 4, all diluted samples yielded low quality (0-10% motility). During the freezing experiments, the highest post-thaw motility rates were observed when sperm was cryopreserved in methylglycol (76-88%), compared to DMSO (23-59%), and NaHCO3 1% was a better activation medium than NaCl 0.29% only when DMSO was used as cryoprotectant. Extenders and thawing temperatures had no effect on post-thaw sperm motility. None of the extenders tested prolonged sperm quality of piabanha beyond two days of storage. On the other hand, sperm can be 1 Advisor: Profa. Ana Tereza de Mendonca Viveiros-Leal – UFLA. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.ufla.br/jspui/bitstream/1/2597/1/DISSERTA%C3%87%C3%83O_Resfriamento%20e%20criopreserva%C3%A7%C3%A3o%20de%20s%C3%AAmen%20de%20peixe%20tele%C3%B3steo%20Piabanha%20(Brycon%20insignis).pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
