NDLI: Antiproliferative and differentiative effect of granulocyte-macrophage colony-stimulating factor on a variant human small cell lung cancer cell line.

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Antiproliferative and differentiative effect of granulocyte-macrophage colony-stimulating factor on a variant human small cell lung cancer cell line.

Content Provider	Semantic Scholar
Author	Yamashita, Yasuyuki Nara, Nobuo Aoki, Naoko Tabei
Copyright Year	1989
Abstract	A variant clone was adopted during passages of a small cell lung cancer cell line, GKT3-1.3. The variant clone exhibited distinct characteristics with alterations in morphology, positive staining with nonspecific esterase stain, and an increase in surface specific markers OKM5, HLA-DR, Mo1, and My7, usually found on monocytes or their precursors. However, it exerted a very rapid proliferation just like immature cells. This new clone, GKT3-1.3V, was shown to have specific binding capacity to granulocyte-macrophage colony-stimulating factor (GM-CSF), with a number of binding sites comparable to that of myelomonocytes or monocytic cell lines. Thus its proliferation was inhibited by GM-CSF in clonogenic assay and suspension culture. Increase in the percentage of cells with surface marker Mo1 by the addition of GM-CSF suggested its differentiative effect. Cell cycle analysis showed that the antiproliferative effect of GM-CSF was due to a block in G0 or G1. The antiproliferative effect of GM-CSF was abolished by the addition of anti-GM-CSF antibody.
Starting Page	49
Ending Page	52
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/canres/49/19/5334.full.pdf
PubMed reference number	2548718v1
Volume Number	49
Issue Number	19
Journal	Cancer research
Language	English
Access Restriction	Open
Subject Keyword	Binding Sites Clone Entity Name Part Qualifier - adopted Esterases F Factor Gentamicins Granulocyte-Macrophage Colony-Stimulating Factor HLA-DR Antigens Leukemia, B-Cell Monocytes Recombinant Granulocyte-Macrophage Colony-Stimulating Factors Small cell carcinoma of lung Staining method Stimulation (motivation) Suspension Culture cancer cell carboxylesterase granulocyte
Content Type	Text
Resource Type	Article

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