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Fluorescence Molecular Imaging and Tomography of Matrix Metalloproteinase-Activatable Near-Infrared Fluorescence Probe and Image-Guided Orthotopic Glioma Resection
| Content Provider | Semantic Scholar |
|---|---|
| Author | Du, Yang Chen, Xinjian Tian, Jie |
| Copyright Year | 2017 |
| Abstract | PurposeMalignant gliomas are major causes of cancer-related mortality and morbidity. Traditional surgery usually leads to incomplete resection of gliomas resulting in the high incidence of tumor recurrence. Advanced medical imaging technology, such as fluorescence imaging-guided surgery, combined with tumor-specific imaging probes allows the identification of tumor margins and improved surgery. However, there are two pressing issues that need to be addressed: first, few fluorescence imaging probes can specifically target gliomas; second, fluorescence molecular imaging (FMI) cannot get the in-depth information of deep-seated gliomas; both of which affect the complete removal of the gliomas.ProceduresIn this study, the biodistribution of smart matrix metalloproteinase (MMP) targeting near-infrared (NIR) fluorescent probe MMPSense 750 FAST (MMP-750) was examined in both U87MG-GFP-fLuc glioma xenograft and orthotopic mouse models using FMI. Then, CT and FMI images of orthotopic gliomas were acquired for the reconstruction of fluorescence molecular tomography (FMT) using a randomly enhanced adaptive subspace pursuit (REASP) algorithm. Furthermore, the resection of orthotopic glioma was performed using the fluorescence surgical navigation system after the injection of the MMP-750 probe. After surgery, bioluminescence imaging (BLI) and hematoxylin and eosin staining were carried out to confirm the precision resection of the tumor.ResultsFMI results showed that the MMP-750 probe can specifically target U87MG glioma in vivo. FMT presented the spatial information of the orthotopic glioma using the REASP reconstruction algorithm. Furthermore, MMP-750 could effectively delineate the tumor margin during glioma surgery leading to a complete resection of the tumors.ConclusionsThe smart MMP-750 specifically targets the glioma and FMT of MMP-750 provides 3D information for the spatial localization of the glioma. MMP-750 can work as an ideal fluorescence probe for guiding the intraoperative surgical resection of the glioma, possessing clinical translation. |
| Starting Page | 930 |
| Ending Page | 939 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11307-017-1158-7 |
| PubMed reference number | 29651576 |
| Journal | Medline |
| Volume Number | 20 |
| Alternate Webpage(s) | http://www.mipav.net/MIPAV%20Papers/lili%E8%AE%BA%E6%96%87%E8%A7%81%E5%88%8A.pdf |
| Journal | Molecular Imaging and Biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
