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First report of a leaf spot of sweet basil (Ocimum basilicum) caused by Alternaria alternata in Italy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Garibaldi, Angelo Gilardi, Giovanna Bertoldo, Costanzo Gullino, Maria Lodovica |
| Copyright Year | 2011 |
| Abstract | Sweet basil (Ocimum basilicum L.) is a popular crop in Italy. During summer-fall 2010, extensive necroses were observed on the leaves of plants grown both in soilless systems and soil in Piedmont (northern Italy). The disease affected 10% of 60-day-old soilless-grown plants and 40% of 5-month-old soil-grown plants. Brown-black lesions often surrounded by a yellow halo developed from the margins and tips of the upper side of older leaves, leading to progressive defoliation of the plants, rarely followed by plant death. A fungus, consistently isolated on PDA from symptomatic leaves, formed conidia singly or in short chains (2-8 elements), dark brown, with 3-7 transverse and 0-4 longitudinal septa, 23.7-73.4×8.8-15.1 μm in size, and with a conical or cylindrical beak 3.5-19.4 μm long. The pathogen was identified as Alternaria sp. based on morphology (Simmons, 2007). The ITS region of rDNA was amplified using ITS1/ITS4 primers and sequenced. BLAST analysis of a 523 bp segment confirmed that the sequence corresponded to A. alternata (GenBank accession No. HQ540552). Two pathogenicity tests were performed in greenhouse at temperatures between 23 and 26°C by spraying leaves of healthy 40-day-old sweet basil plants cv. Genovese gigante with a 105 CFU/ml spore suspension. Plants sprayed only with water served as controls. Lesions resembling those observed in the field developed on the leaves 7 days post inoculation, whereas control plants remained healthy. A. alternata was consistently reisolated from artificially inoculated plants. A. alternata has been reported on basil from Japan (Taba et al., 2009). This is the first report from Italy and, to the best of our knowledge, also from Europe. |
| Starting Page | 4 |
| Ending Page | 71 |
| Page Count | 68 |
| File Format | PDF HTM / HTML |
| DOI | 10.4454/jpp.v93i4.2376 |
| Volume Number | 93 |
| Alternate Webpage(s) | http://www.sipav.org/main/jpp/index.php/jpp/article/download/2376/1053 |
| Alternate Webpage(s) | https://doi.org/10.4454/jpp.v93i4.2376 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
