Self-assembled biosensor with universal reporter and dual-quenchers for detection of unlabelled nucleic acids.

Content Provider	Semantic Scholar
Author	Huang, Liming Aryal, Gyan Hari Tam-Chang, Suk-Wah Publicover, Nelson G. Hunter, Kenneth W.
Copyright Year	2016
Abstract	A novel biosensor with universal reporter and dual quenchers was developed for rapid, sensitive, selective, and inexpensive detection of unlabelled nucleic acids. The biosensor is based on a single-strand DNA stem-loop motif with an extended universal reporter-binding region, a G-base rich stem region, and a universal address-binding region. The self-assembly of these stem-loop probes with fluorescence labeled universal reporter and a universal address region conjugated to gold nanoparticles forms the basis of a biosensor for DNA or microRNA targets in solution. The introduction of dual quenchers (G-base quenching and gold surface plasmon resonance-induced quenching) significantly reduces the fluorescence background to as low as 12% of its original fluorescence intensity and hence enhances the detection limit to 0.01 picomoles without signal ampilication.
File Format	PDF HTM / HTML
DOI	10.1039/c5an02094h
PubMed reference number	26757447
Journal	Medline
Volume Number	141
Issue Number	4
Alternate Webpage(s)	http://www.rsc.org/suppdata/c5/an/c5an02094h/c5an02094h1.pdf
Alternate Webpage(s)	http://pubs.rsc.org/en/content/getauthorversionpdf/C5AN02094H
Alternate Webpage(s)	https://doi.org/10.1039/c5an02094h
Journal	The Analyst
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article