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Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Giesy, John Paul Hilscherová, Klára Jones, P. D. Kannan, Kurunthachalam Machala, Miroslav |
| Copyright Year | 2002 |
| Abstract | In vitro cell bioassays are useful techniques for the determination of receptor-mediated activities in environmental samples containing complex mixtures of contaminants. The cell bioassays determine contamination by pollutants that act through specific modes of action. This article presents strategies for the evaluation of aryl hydrocarbon receptor (hereafter referred as dioxin-like) or estrogen receptor mediated activities of potential endocrine disrupting compounds in complex environmental mixtures. Extracts from various types of environmental or food matrices can be tested by this technique to evaluate their 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents or estrogenic equivalents and to identify contaminated samples that need further investigation using resource-intensive instrumental analyses. Fractionation of sample extracts exhibiting significant activities, and subsequent reanalysis with the bioassays can identify important classes of contaminants that are responsible for the observed activity. Effect-directed chemical analysis is performed only for the active fractions to determine the responsible compounds. Potency-balance estimates of all major compounds contributing to the observed effects can be calculated to determine if all of the activity has been identified, and to assess the potential for interactions such as synergism or antagonism among contaminants present in the complex mixtures. The bioassay approach is an efficient (fast and cost effective) screening system to identify the samples of interest and to provide basic information for further analysis and risk evaluation. |
| Starting Page | 3 |
| Ending Page | 16 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0025-326X(02)00097-8 |
| PubMed reference number | 12398362 |
| Journal | Medline |
| Volume Number | 45 |
| Issue Number | 1-12 |
| Alternate Webpage(s) | http://www.usask.ca/toxicology/jgiesy/pdf/publications/JA-327.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S0025-326X%2802%2900097-8 |
| Journal | Marine pollution bulletin |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
