NDLI: Dimerization of P-Selectin Glycoprotein Ligand-1 (PSGL-1) Required for Optimal Recognition of P-Selectin

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Dimerization of P-Selectin Glycoprotein Ligand-1 (PSGL-1) Required for Optimal Recognition of P-Selectin

Content Provider	Semantic Scholar
Author	Snapp, Karen R. Craig, Ron Herron, Michael Nelson, R. David Stoolman, Lloyd M. Kansas, Geoffrey S.
Copyright Year	1998
Abstract	Interactions between P-selectin, expressed on endothelial cells and activated platelets, and its leukocyte ligand, a homodimer termed P-selectin glycoprotein ligand-1 (PSGL-1), mediate the earliest adhesive events during an inflammatory response. To investigate whether dimerization of PSGL-1 is essential for functional interactions with P-selectin, a mutant form of PSGL-1 was generated in which the conserved membrane proximal cysteine was mutated to alanine (designated C320A). Western blotting under both denaturing and native conditions of the C320A PSGL-1 mutant isolated from stably transfected cells revealed expression of only a monomeric form of PSGL-1. In contrast to cells cotransfected with alpha1-3 fucosyltransferase-VII (FucT-VII) plus PSGL-1, K562 cells expressing FucT-VII plus C320A failed to bind COS cells transfected with P-selectin in a low shear adhesion assay, or to roll on CHO cells transfected with P-selectin under conditions of physiologic flow. In addition, C320A transfectants failed to bind chimeric P-selectin fusion proteins. Both PSGL-1 and C320A were uniformly distributed on the surface of transfected K562 cells. Thus, dimerization of PSGL-1 through the single, conserved, extracellular cysteine is essential for functional recognition of P-selectin.
Starting Page	263
Ending Page	270
Page Count	8
File Format	PDF HTM / HTML
PubMed reference number	9660879v1
Volume Number	142
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/142/1/263.full.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/ad/aa/12596.PMC2133017.pdf
Language	English
Access Restriction	Open
Subject Keyword	Adhesives Alanine Blood Platelets Chimera organism Dimerization Fucosyltransferase K-562 Leukocytes Ligands P-Selectin SELP gene Selectins Tissue membrane Western Blotting alpha(1,2)-L-fucosyltransferase activity glycoprotein 6-alpha-L-fucosyltransferase activity inflammatory response
Content Type	Text
Resource Type	Article

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