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Evaluation of headspace Solid Phase Micro-extraction method for analysis of phosphine residues in wheat
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 2010 |
| Abstract | This new method utilizes headspace-solid-phase micro extraction (HS-SPME) for pre-concentration of PH3. Phosphine was determined with gas chromatography/pulsed flame photometric detector (PFPD). Spiked samples were used for calculation of phosphine residue in grain. Four types of fibres (100μmPDMS, 85μm-CAR/PDMS, 75μm-CAR/PDMS and 65μm-PDMS/DVB) were tested. The bipolar fibres (CAR/PDMS and PDMS/DVB) can extract PH3, but the non-polar fibre (PDMS) did not. Larger size fibres extracted PH3 more efficiently than the smaller size fibres (e.g., 85 μm > 75 μm > 65 μm). The 85μm CAR/PDMS fibre was used to optimize the different parameters that affect the SPME extraction efficiency of PH3. In the validation study, 50 grams of wheat in a 250 mL glass flask and capped with an open-top screw cap and PTFE/Silicon septa were spiked at 0.02 ng PH3/g of wheat. The flask was then heated to 45°C in an oil bath for 45 min, after which time the 85 μm CAR/PDMS fibre was exposed for 20 min and then exposed in the heated injection port of a GC/PFPD and desorbed for 2 min. Under conditions of the validation study, the limit of detection (LOD) or level of quantification (LOQ) was in the range of 0.005–0.01 ng PH3/g of wheat. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://researchrepository.murdoch.edu.au/id/eprint/4374/1/10th_IWCSPP_SPME_-_PH3_2010_final_ppt.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
