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Large-scale production of CMP-NeuAc and sialylated oligosaccharides through bacterial coupling
| Content Provider | Semantic Scholar |
|---|---|
| Author | Endo, Tetsuo Koizumi, S. Tabata, Kazuhiko Ozaki, Akio |
| Copyright Year | 2000 |
| Abstract | Abstract A large-scale production system of cytidine 5′-monophospho-N-acetylneuraminic acid (CMP-NeuAc) and sialyloligosaccharides was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. For the production of CMP-NeuAc, two recombinant E. coli strains were generated that overexpressed the genes of CMP-NeuAc synthetase and CTP synthetase, respectively. C. ammoniagenes contributed to the formation of UTP from orotic acid. CMP-NeuAc was accumulated at 27 mM (17 g/l) after a 27-h reaction starting with orotic acid and N-acetylneuraminic acid. When E. coli cells that overexpressed the α-(2 → 3)-sialyltransferase gene of Neisseria gonorrhoeae were put into the CMP-NeuAc production system, 3′-sialyllactose was accumulated at 52 mM (33 g/l) after an 11-h reaction starting with orotic acid, N-acetylneuraminic acid, and lactose. Almost no oligosaccharide byproducts other than 3′-sialyllactose were observed after the reaction. The production of 3′-sialyllactose at a 5-l jar fermenter scale was almost the same as that at a beaker scale, which indicated the high potential of the 3′-sialyllactose production on an industrial scale. |
| Starting Page | 257 |
| Ending Page | 261 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s002530050017 |
| PubMed reference number | 10772462 |
| Journal | Medline |
| Volume Number | 53 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s002530050017 |
| Journal | Applied Microbiology and Biotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
