NDLI: Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.

Please wait, while we are loading the content...

Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.

Content Provider	Semantic Scholar
Author	Farkas, Joel Chung, Daehwan DeBarry, Megan B. Adams, Michael Wayne Westpheling, Janet
Copyright Year	2011
Abstract	We report the construction of a series of replicating shuttle vectors that consist of a low-copy-number cloning vector for Escherichia coli and functional components of the origin of replication (oriC) of the chromosome of the hyperthermophilic archaeon Pyrococcus furiosus. In the process of identifying the minimum replication origin sequence required for autonomous plasmid replication in P. furiosus, we discovered that several features of the origin predicted by bioinformatic analysis and in vitro binding studies were not essential for stable autonomous plasmid replication. A minimum region required to promote plasmid DNA replication was identified, and plasmids based on this sequence readily transformed P. furiosus. The plasmids replicated autonomously and existed in a single copy. In contrast to shuttle vectors based on a plasmid from the closely related hyperthermophile Pyrococcus abyssi for use in P. furiosus, plasmids based on the P. furiosus chromosomal origin were structurally unchanged after transformation and were stable without selection for more than 100 generations.
Starting Page	564
Ending Page	567
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://bioenergycenter.org/besc/publications/farkas_defining.pdf
PubMed reference number	21784908v1
Alternate Webpage(s)	https://doi.org/10.1128/AEM.05057-11
DOI	10.1128/AEM.05057-11
Journal	Applied and environmental microbiology
Volume Number	77
Issue Number	18
Language	English
Access Restriction	Open
Subject Keyword	Archaea Bio-Informatics DNA Replication Plasmids Replication Origin hyperthermophile red tungsten protein, Pyrococcus furiosus
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Defining Components of the Chromosomal Origin of Replication of the Hyperthermophilic Archaeon Pyrococcus furiosus Needed for Construction of a Stable Replicating Shuttle Vector ▿ †

Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon.

Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon pyrococcus abyssi (2002).

DNA replication in thermophiles.

Defining genes in the genome of the hyperthermophilic archaeon pyrococcus furiosus: implications for all microbial genomes† (2005).

Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus

Protein repair methyltransferase from the hyperthermophilic archaeon Pyrococcus furiosus. Unusual methyl-accepting affinity for D-aspartyl and N-succinyl-containing peptides.

Characterization of an aminoacylase from the hyperthermophilic archaeon Pyrococcus furiosus (2001)

Rubrerythrin from the hyperthermophilic archaeon pyrococcus furiosus is a rubredoxin-dependent, (2004).

Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.

Similar Documents

Defining Components of the Chromosomal Origin of Replication of the Hyperthermophilic Archaeon Pyrococcus furiosus Needed for Construction of a Stable Replicating Shuttle Vector ▿ †

Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon.

Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon pyrococcus abyssi (2002).

DNA replication in thermophiles.

Defining genes in the genome of the hyperthermophilic archaeon pyrococcus furiosus: implications for all microbial genomes† (2005).

Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus

Protein repair methyltransferase from the hyperthermophilic archaeon Pyrococcus furiosus. Unusual methyl-accepting affinity for D-aspartyl and N-succinyl-containing peptides.

Characterization of an aminoacylase from the hyperthermophilic archaeon Pyrococcus furiosus (2001)

Rubrerythrin from the hyperthermophilic archaeon pyrococcus furiosus is a rubredoxin-dependent, (2004).

Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.