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Alpha-2 adrenergic agonism stimulates islet glucagon release from perfused rat pancreas: possible involvement of alpha-2A adrenergic receptor subtype.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hirose, Hiroshi Maruyama, Hiroshi Itoh, Kazuto Koyama, Katsuhiro Kido, Koichiro Saruta, Takao |
| Copyright Year | 1992 |
| Abstract | Although insulin release is known to be inhibited by alpha-2 adrenergic agonism, the effect of alpha adrenergic agonism on islet glucagon release remains controversial. Alpha-2 adrenoceptors are subdivided into alpha-2A and alpha-2B subtypes using receptor binding methods or cloning methodology. This study was designed to confirm the involvement of the alpha-2 adrenoceptor and its subtypes in glucagon release from the isolated, perfused rat pancreas. Both the alpha-2A preferential agonist oxymetazoline and the non-subtype-selective alpha-2 agonist clonidine induced concentration-dependent stimulation of glucagon release, starting at 10(-8) and 10(-7) mol/l, respectively (p < 0.01). In contrast, neither of the two alpha-1 selective agonists, methoxamine and phenylephrine, at concentrations up to 10(-6) mol/l affected glucagon release. Furthermore, the non-subtype-selective alpha-2 antagonist rauwolscine at concentrations of 10(-6) and 10(-5) mol/l and the alpha-1 and alpha-2A selective antagonist WB-4101 at 10(-5) mol/l showed significant antagonism of 10(-7) mol/l clonidine-induced glucagon release versus corresponding controls. Neither the alpha-1 and alpha-2B selective antagonist prazosin nor the alpha-2B preferential antagonist chlorpromazine, at concentrations up to 10(-5) mol/l, antagonized the effects of clonidine. None of the eight drugs, at the concentrations tested, affected insulin release with 5.5 mmol/l glucose. These results suggest that in rats islet glucagon release induced by alpha adrenoceptor agonism is mediated through alpha-2 adrenoceptors, possibly the alpha-2A subtype. |
| File Format | PDF HTM / HTML |
| DOI | 10.1530/acta.0.1270279 |
| PubMed reference number | 1357904 |
| Journal | Medline |
| Volume Number | 127 |
| Issue Number | 3 |
| Alternate Webpage(s) | https://eje.bioscientifica.com/downloadpdf/journals/eje/127/3/acta_127_3_017.pdf |
| Alternate Webpage(s) | https://doi.org/10.1530/acta.0.1270279 |
| Journal | Acta endocrinologica |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
