Loading...
Please wait, while we are loading the content...
Similar Documents
Quantifying the Surface Coverage of Conjugate Molecules on Functionalized Nanoparticles
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pease, Leonard F. Tsai, De-Hao Zangmeister, Rebecca A. Zachariah, Michael R. Tarlov, Michael J. |
| Copyright Year | 2007 |
| Abstract | Here we present a method to determine the surface coverage or surface density of biological molecules conjugated to nanoparticle surfaces. Electrospray-differential mobility analysis (ES-DMA) is used to determine a coating thickness by measuring the change in the size of gold nanoparticles before and after modification with thiol-derivatized single-stranded DNA. The DNA surface coverage is then obtained from the coating thickness through the use of a simple random coil model. The method requires neither fluorescent tagging nor calibration curves. We believe ES-DMA to be a broadly applicable nanometrology tool for the characterization of biologically conjugated nanoparticles. |
| Starting Page | 17155 |
| Ending Page | 17157 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/jp075571t |
| Volume Number | 111 |
| Alternate Webpage(s) | http://www.mrzgroup.umd.edu/pdfs/2007_JPC_DNAongold.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/jp075571t |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
