Addition of telomere-associated HeT DNA sequences “heals” broken chromosome ends in Drosophila

Content Provider	Semantic Scholar
Author	Biessmann, Harald Mason, James Michael Ferry, Kristian D'Hulst, Marie Pardue, M. L.
Copyright Year	1990
Abstract	Stocks of D. melanogaster X chromosomes carrying terminal deletions (RT chromosomes) have been maintained for several years. Some of the chromosomes are slowly losing DNA from the broken ends (as expected if replication is incomplete) and show no telomere-associated DNA added to the receding ends. Two stocks carry chromosomes that have become "healed" and are no longer losing DNA. In both stocks the broken chromosome end has acquired a segment of HeT DNA, a family of complex repeats found only at telomeres and in pericentric heterochromatin. Although the HeT family is complex, the HeT sequence joined to the broken chromosome end is the same in both stocks. In contrast, the two chromosomes are broken in different places and have no detectable sequence similarity at the junction with the new DNA. Sequence analysis suggests that the new telomere sequences have been added by a specific mechanism that does not involve homologous recombination.
Starting Page	663
Ending Page	673
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1016/0092-8674(90)90478-W
PubMed reference number	2111731
Journal	Medline
Volume Number	61
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/009286749090478W
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/009286749090478W?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/0092-8674%2890%2990478-W
Journal	Cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article