NDLI: Isolation and cross-species amplification of microsatellite loci in the Siberian jay (Perisoreus infaustus).

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Isolation and cross-species amplification of microsatellite loci in the Siberian jay (Perisoreus infaustus).

Content Provider	Semantic Scholar
Author	Lillandt, Bo-Göran Bensch, Staffan Hansson, Bengt Wennerberg, Liv Schantz, Torbjörn Von
Copyright Year	2002
Abstract	Microsatellites are superior compared to other genetical markers for parentage determination, because they can be analysed from tiny and partially degraded DNA-samples extracted from e.g. hairs or bird feathers (ELLEGREN 1992). However, bird genomes contain relatively few microsatellite loci (LONGMIRE et al. 1999; PRIMMER et al. 1997b). It is therefore a tedious process to isolate a set of markers that is sufficient for conclusive parentage analyses. Here we report on nine microsatellite markers that are polymorphic in the Siberian jay (Perisoreus infaustus), a resident family dwelling species occurring throughout the Eurasian taiga (HELLE and LILLANDT 1997). The markers were found using two methods; (1) isolating new microsatellite sequences from a size-selected Siberian jay genomic library, and (2) exploring 64 heterologous microsatellite markers isolated in other mostly passerine species, and modifying primer sequences if necessary. This set of nine partly unpublished markers have previously been used for parentage testing on 298 juvenile Siberian jays from which feather or blood samples were collected during a long-term study in Finland 1976–1998. Methods for data analysis and parentage determinations were described in LIL-
File Format	PDF HTM / HTML
DOI	10.1034/j.1601-5223.2002.01675.x
Alternate Webpage(s)	http://portal.research.lu.se/ws/files/2962553/624654.pdf
Alternate Webpage(s)	http://portal.research.lu.se/portal/files/2962553/624654.pdf
Alternate Webpage(s)	http://lup.lub.lu.se/search/ws/files/2962553/624654.pdf
Alternate Webpage(s)	http://retki.pp.fi/dark/bgl_br02.pdf
PubMed reference number	12627843
Alternate Webpage(s)	https://doi.org/10.1034/j.1601-5223.2002.01675.x
Journal	Medline
Volume Number	137
Issue Number	2
Journal	Hereditas
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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