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Ultrasensitive electrochemical DNA detection based on dual amplification of circular strand-displacement polymerase reaction and hybridization chain reaction.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Cui Zhou, Hui Zhu, Wenping Li, Hongbo Jiang, Jianhui Shen, Guoli Yu, Ruqin |
| Copyright Year | 2013 |
| Abstract | We developed a novel electrochemical strategy for ultrasensitive DNA detection using a dual amplification strategy based on the circular strand-displacement polymerase reaction (CSDPR) and the hybridization chain reaction (HCR). In this assay, hybridization of hairpin-shaped capture DNA to target DNA resulted in a conformational change of the capture DNA with a concomitant exposure of its stem. The primer was then hybridized with the exposed stem and triggered a polymerization reaction, allowing a cyclic reaction comprising release of target DNA, hybridization of target with remaining capture DNA, polymerization initiated by the primer. Furthermore, the free part of the primer propagated a chain reaction of hybridization events between two DNA hairpin probes with biotin labels, enabling an electrochemical reading using the streptavidin-alkaline phosphatase. The proposed biosensor showed to have very high sensitivity and selectivity with a dynamic response range through 10fM to 1nM, and the detect limit was as low as 8fM. The proposed strategy could have the potential for molecular diagnostics in complex biological systems. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.bios.2013.03.020 |
| PubMed reference number | 23603128 |
| Journal | Medline |
| Volume Number | 47 |
| Alternate Webpage(s) | http://or.nsfc.gov.cn/bitstream/00001903-5/26529/1/1000006777313.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.bios.2013.03.020 |
| Journal | Biosensors & bioelectronics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
