Loading...
Please wait, while we are loading the content...
Similar Documents
Water deficit-related expressed sequence tags (ESTS) in the sugarcane (Saccharum spp.) leaf roll.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ferro, Maria Inês T. Noda, Roberto Willians Tezza, R. I. D. Jovino, D. F. R. Dabbas, Karina Maia Zingarette, S. M. Suzuki, Jun-Ichiro |
| Copyright Year | 2007 |
| Abstract | WATER STRESS is one of the most common environmental stresses which affect plant productivity and their geographic distribution. Plants have mechanisms for water stress adaptation that might be characterised by the accumulation of specific mRNAs as the result of specific gene activation. In this work, expressed sequence tags (ESTs) from sugarcane leaf roll submitted to water deficit stress were analysed. Two cDNA libraries were constructed from plants subjected to 9 and 17 days of water stress. A total of 4639 sequences with good quality (minimum of 100 bases and score 20 according to the quality analysis of Phred program) were clustered using the CAP3 program. Among the clusters, 2077 ESTs were singletons and 2405 ESTs were in 905 clusters containing from 2 to 21 ESTs. Putative regulatory and metabolic proteins related to sugarcane water stress were identified from these 2982 unique transcripts (clusters + singletons) analysed by a similarity search using the NCBI BLASTX algorithm against nonredundant NCBI BLAST protein databases. Based on the NCBI TBLASTX algorithm, 188 unique transcripts were found. These unique transcripts were not similar to the unique transcripts reported by the Sugarcane EST Project from 'Genoma FAPESP' Program (SUCEST http://sucest.lad.dcc.unicamp.br/en/), even though these results were obtained from plants grown under different environmental conditions. An analysis of the highest unique transcription frequencies data revealed a set of genes expressing metabolic enzymes such as a lipoxygenase, S-adenosylmethionine synthetase, pyrophosphate-fructose-6-phosphate 1-phosphotransferase and sucrose synthetase. All these enzymes are strictly expressed and appear to be highly related to evolution of drought stress, underlying novelties in sugarcane drought research. |
| Starting Page | 953 |
| Ending Page | 957 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.issct.org/pdf/proceedings/2007/Papers/2007%20Ferro,%20WATER%20DEFICIT-RELATED%20EXPRESSED%20SEQUENCE%20TAGS%20(ESTS)%20IN%20THE%20SUGARCANE%20(SACCHARUM%20SPP..pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
