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Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nikaido, I. Asamizu, Erika Nakajima, M. Nakamura, Y. Saga, Naotsune Tabata, Satoshi |
| Copyright Year | 2000 |
| Abstract | A total of 10,154 5'-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanobacteria, while 2187 (66.9%) are novel. Codon usage analysis in the coding regions of 101 non-redundant EST groups showing significant similarity to known genes indicated the higher GC contents at the third position of codons (79.4%) than the first (62.2%) and the second position (45.0%), suggesting that the genome has been exposed to high GC pressure during evolution. The sequence data of individual ESTs are available at the web site http://www.kazusa.or.jp/en/plant/porphyra/EST/. |
| File Format | PDF HTM / HTML |
| DOI | 10.1093/dnares/7.3.223 |
| PubMed reference number | 10907854 |
| Journal | Medline |
| Volume Number | 7 |
| Issue Number | 3 |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/dnaresearch/7/3/10.1093_dnares_7.3.223/3/dnares_7_3_223.pdf?Expires=1498073543&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=dTaAfIvd3Ljcl6rn6qvf9HK0VPvvJ5ZM~8oPKkM2Inp7aY8YJ4j-zv2BR2u2F-s5vZ5MXYFLR0f2hmjoVOHK4eHhwgubhvB12-z4alsztINNHoAOogc0X30ch6LsrBHyaV2w8D3XRpGjUQYXuPRONTXCsPA2~OZo5q-5NU60DkAggpbSWIT-Krb0ouqVYbrOTi-pOhxKqhCIjDy73Ut9gqfZRHm8yli8R90fHScLyxFaXWfdlrF0fsXrkbA6b4TjeH79JpHVettXNfgNRfxS0FlRmMOmHhY5dvxNe8Zky9n~33gR1ljHt2WhEKO4BWMkBZBWko5TD8rj08Mv02rHIg__ |
| Alternate Webpage(s) | https://watermark.silverchair.com/dnares_7_3_223.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbYwggGyBgkqhkiG9w0BBwagggGjMIIBnwIBADCCAZgGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM5HxFr03FtN_UFhXjAgEQgIIBafdKonVc2ZdzvC80qIcz22lwxYlaSOXgLjFBxaA223vTCEtQsJrBsWl1CggLUV-jeLFCerXTRjtQ60pU2RCGF5J-M4Vo-FziGDWtXaiuzkvD3EmioN2nFEXuo5Ru4FRCY1CHecHtuQH0FLU8N-VnEF47LyArNUlUEVxwecOQJbGAGzG_6A0EtYFXeZX2u2YjPp2jh9f0uB7jLV7ZxHxJvJR_XOA4ddSVEIfEzunENU3fZNy0vJNGUR7-fPHLl1aHsqnJZnpfie9VvVG_gUN1zBtBqF7noIODcbMtVTwgDP9YmENgtTD5gCxhDR2KXgt2UXNCSEYvFmNAmshNio5Vl_oKVlpZjQvv-FV3niuKrsLfLVVxwYyEqDD1PVLVOHS-Uk7TpNGRQRfOjoFzbPDE5-y8hgNkIzivw760b5nM_qbdy4oOJywFn9ugDxG57J6hPOwpcRNWfvYK80zxl_cAmjrSvJgehrZnfwk |
| Alternate Webpage(s) | https://doi.org/10.1093/dnares%2F7.3.223 |
| Journal | DNA research : an international journal for rapid publication of reports on genes and genomes |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |