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Simultaneous quantification of peptides and phosphopeptides by capLC-ICP-MS using the Agilent 8800 / 8900 Triple Quadrupole ICP-MS Application note
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fernández, Silvia Diez Sugiyama, Naoki Encinar, Jorge Ruiz Sanz-Medel, Alfredo |
| Copyright Year | 2016 |
| Abstract | In combination with capillary LC (capLC) separation, the Agilent 8800 Triple Quadrupole ICP-MS, operating in MS/MS mass shift mode, was used for the trace measurement of phosphorus (P) in phosphopeptides and sulfur (S) in S-containing peptides. The lowest absolute detection limits ever reported for the analysis of Sand P-containing species (11 fmol and 6.6 fmol, respectively) using LC-ICP-MS were achieved. Effective interference removal was confirmed by good agreement with the theoretical isotope ratio for S. Excellent peak profiles and signal to noise (S/N) ratios were observed for both P and S. The great potential of triple quadrupole capLC-ICP-MS for sensitive and simultaneous absolute quantitation of Pand S-containing peptides using non-specific standards is demonstrated. Simultaneous quantification of peptides and phosphopeptides by capLC-ICPMS using the Agilent 8800/8900 Triple Quadrupole ICP-MS Application note |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.agilent.com/cs/library/applications/5991-1461EN.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
