NDLI: An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli

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An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli

Content Provider	Semantic Scholar
Author	Zhang, De Yuan Zhou, Jin Yun Fang, Jinbo Wu, Jemma X. Mu, Anandit
Copyright Year	2018
Abstract	The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in China was expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay ( N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure.
Starting Page	336
Ending Page	344
Page Count	9
File Format	PDF HTM / HTML
DOI	10.17221/5632-vetmed
Alternate Webpage(s)	http://www.agriculturejournals.cz/publicFiles/61307.pdf
Alternate Webpage(s)	http://vri.cz/docs/vetmed/50-8-336.pdf
Alternate Webpage(s)	https://doi.org/10.17221/5632-vetmed
Volume Number	50
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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