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Protein Dye Affinity Chromatography Using Immobilized
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tetraiodofluorescein Tucker, Ronald F. Babul, Jorge Stellwagen, Earle |
| Copyright Year | 2001 |
| Abstract | The chromatographic behavior of a heterogeneous protein mixture and of a series of homogeneous proteins on the immobilized dye tetraiodofluorescein has been observed and analyzed. Less than 656, of the millimolar concentration of dye immobilized to a porous agarose matrix is accessible to protein. The affinity of a protein for immobilized dye is dramatically increased by insertion of apolar spacer atoms between the dye and the matrix. Dye columns constructed with a 9-atom spacer can be used to advantage for the retention and competitive elution of proteins not found previously amenable to dye chromatography. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/256/21/10993.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
