NDLI: Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances.

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Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances.

Content Provider	Semantic Scholar
Author	Truskey, G. A. Burmeister, Jörg Grapa, E. Reichert, William M.
Copyright Year	1992
Abstract	A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were collected of bovine aortic endothelial cells (BAEC) plated onto glass microscope slides for 15 min, 30 min and 24 h. BAEC adherent for 15 min showed an absence of a focal contact morphology, with the region of closest apposition beneath the cell center. After 30 min, multiple contacts with the surface were established and the morphology became more irregular. BAEC attached for 24 h showed well-defined focal contact regions aligned in characteristically striated patterns. The relative distance between closest and farthest membrane/substratum separations are consistent with reported distance between focal and matrix contacts. Topographical maps of membrane/substratum separation distances over the entire ventral surface of the plated cells were constructed to demonstrate the utility of quantitative TIRF microscopy.
File Format	PDF HTM / HTML
PubMed reference number	1478950
Journal	Medline
Volume Number	103
Part	2
Alternate Webpage(s)	http://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?amp%3Bcontext=soecs-facarticles&article=1037
Alternate Webpage(s)	http://jcs.biologists.org/content/joces/103/2/491.full.pdf
Alternate Webpage(s)	https://scholarlycommons.pacific.edu/cgi/viewcontent.cgi?amp%3Bcontext=soecs-facarticles&article=1037&httpsredir=1&referer=
Journal	Journal of cell science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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