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Econazole attenuates cytotoxicity of 1-methyl-4-phenylpyridinium by suppressing mitochondrial membrane permeability transition
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lee, Chung Soo Song, Jin Ho Han, Eun Sook |
| Copyright Year | 2006 |
| Abstract | Defects in mitochondrial function have been shown to participate in the induction of neuronal cell injury. The effect of econazole against the cytotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells was assessed in relation to the mitochondrial membrane permeability changes. Treatment of PC12 cells with MPP(+) resulted in the nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome c, activation of caspase-3, increase in the formation of reactive oxygen species (ROS) and depletion of GSH. Econazole (0.25-2.5 microM) inhibited the cytotoxicity of MPP(+) or rotenone. The addition of econazole (0.5 microM) significantly attenuated the MPP(+)-induced mitochondrial damage, elevation of intracellular Ca(2+) level and cell death. However, because of the cytotoxicity, econazole at 5 microM did not attenuate the toxicity of MPP(+). The results show that econazole at the low concentrations may reduce the MPP(+)-induced viability loss in PC12 cells by suppressing the mitochondrial permeability transition, leading to activation of caspase-3 and the elevation of intracellular Ca(2+) levels, which are associated with the increased formation of ROS and depletion of GSH. |
| Starting Page | 687 |
| Ending Page | 694 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.brainresbull.2006.03.021 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0361923006001183 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0361923006001183?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 16716839 |
| Alternate Webpage(s) | https://doi.org/10.1016/j.brainresbull.2006.03.021 |
| Journal | Medline |
| Volume Number | 69 |
| Journal | Brain Research Bulletin |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
