NDLI: Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes

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Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes

Content Provider	Semantic Scholar
Author	Rappold, Gudrun A. Cremer, Thomas Hager, H. D. Davies, Kay Elizabeth Mueller, Clemens R. Yang, T.
Copyright Year	2004
Abstract	SummaryTwo cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization experiments. Our data indicate a large variability of the distances between the sex chromosomes in male and female interphase nuclei. However, the mean distance observed between the X and Y chromosome was significantly smaller than the mean distance observed between the two X-chromosomes. The distribution of distances determined experimentally is compared with three model distributions of distances, and the question of a non-random distribution of sex chromosomes is discussed. Mathematical details of these model distributions are provided in an Appendix to this paper. In the case of a human translocation chromosome (Xqter→Xp22.2::Yq11→Y qter) contained in the Chinese hamster x human hybrid cell line 445 x 393, the binding sites of pXBR and CY1 were found close to each other in most interphase nuclei. These data demonstrate the potential use of chromosome-specific repetitive DNA probes to study the problem of interphase chromosome topography.
Starting Page	317
Ending Page	325
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1007/BF00291361
PubMed reference number	6336321
Journal	Medline
Volume Number	67
Alternate Webpage(s)	https://epub.ub.uni-muenchen.de/9270/1/cremer_thomas_9270.pdf
Alternate Webpage(s)	https://doi.org/10.1007/BF00291361
Journal	Human Genetics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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