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Stabilization of tandem dG-dA base pairs in DNA-hairpins: replacement of the canonical bases by 7-deaza-7-propynylpurines.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Seela, Frank Budow, Simone Shaikh, Khalil Isak Jawalekar, Anup M. |
| Copyright Year | 2005 |
| Abstract | The stabilizing effect of 7-propynylated 7-deazapurine nucleosides on DNA-hairpins and DNA-duplexes containing d(GA) mismatches was investigated. The corresponding oligonucleotides were synthesized using solid-phase synthesis. For this purpose, the phosphoramidite of 7-deaza-7-propynyl-2'-deoxyadenosine (3c) was prepared. The incorporation of 3c instead of dA into the tandem d(GA) base pair of a DNA-hairpin alters the secondary structure, but has a positive effect on the duplex stability. A complete replacement of the canonical nucleosides of the tandem d(GA) base pair by 3c and 7-deaza-7-propynyl-2'-deoxyguanosine results in a significant base pair stabilization. |
| Starting Page | 751 |
| Ending Page | 756 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/OB/b5/b510444k/b510444k.pdf |
| PubMed reference number | 16294250v1 |
| Volume Number | 3 |
| Issue Number | 23 |
| Journal | Organic & biomolecular chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Base Pairing Dopamine Oligonucleotides Purine Nucleosides |
| Content Type | Text |
| Resource Type | Article |