Design and development of polymerase chain reaction thermal cycler using proportional-integral temperature controller

Content Provider	Semantic Scholar
Author	Soon, Chong Kim Devi, Nawoor Anusha Gan, Kok Beng Then, Sue-Mian
Copyright Year	2018
Abstract	A thermal cycler is used to amplify segments of DNA using the polymerase chain reaction (PCR). It is an instrument that requires precise temperature control and rapid temperature changes for certain experimental protocols. However, the commercial thermal cyclers are still bulky, expensive and limited for laboratory use only. As such it is difficult for on-site molecular screening and diagnostics. In this work, a portable and low cost thermal cycler was designed and developed. The thermal cycler block was designed to fit six microcentrifuge tubes. A Proportional-Integral temperature controller was used to control the thermal cycler block temperature. The results showed that the maximum temperature ramp rate of the developed thermal cycler was 5.5 °C/s. The proportional gain (K p ) and integral gain (K i ) of the PI controller were 15 A/V and 1.8 A/Vs respectively. Finally, the developed thermal cycler successfully amplified six DNA samples at the expected molecular weight of 150 base pair. It has been validated using the Eppendorf Mastercycler nexus gradient system and gel electrophoresis analysis
Starting Page	213
Ending Page	218
Page Count	6
File Format	PDF HTM / HTML
DOI	10.11113/mjfas.v14n2.765
Volume Number	14
Alternate Webpage(s)	https://mjfas.utm.my/index.php/mjfas/article/download/765/pdf
Alternate Webpage(s)	https://doi.org/10.11113/mjfas.v14n2.765
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article