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Vascular endothelial growth factor, epidermal growth factor and fibroblast growth factor-4 and -10 stimulate trophoblast plasminogen activator system and metalloproteinase-9.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Anteby, Eyal Y. Greenfield, Caryn Natanson-Yaron, Shira Goldman-Wohl, Debra Hamani, Yaron Khudyak, Victoria Ariel, Ilana Yagel, Simcha |
| Copyright Year | 2004 |
| Abstract | Trophoblast invasion, accompanied by degradation of extracellular matrix, is crucial to normal pregnancy development, whereas shallow placental invasion and implantation likely plays a role in the subsequent development of pre-eclampsia. The growth factors vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and fibroblast growth factor (FGF) are placental growth factors that activate degradation of extracellular matrix. We determined the effect of VEGF, EGF, FGF-2, FGF-4 and FGF-10 on the plasminogen activator system of first trimester cytotrophoblasts cultured in vitro. We studied the activity of urokinase plasminogen activator (uPA), its inhibitor plasminogen activator inhibitor-1 (PAI-1), and 92 kDa gelatinase-B (matrix metalloproteinase-9, MMP-9), using protein gel and reversed gel zymography. The expression pattern of FGF-4 and FGF-10 in human placental sections was determined by immunohistochemistry. FGF-4 was expressed in first trimester villi stroma, primarily in endothelial cells. FGF-10 expression was localized to first trimester extravillous trophoblasts. VEGF, EGF, FGF-4 and FGF-10, but not FGF-2, stimulate the activity of trophoblast uPA, PAI-1 and MMP-9. These results support the hypothesis that specific growth factors modulate the invasive potential of trophoblasts, and therefore may play an important role in early placental development. Our findings may contribute to the understanding of the pathophysiology of diseases associated with shallow placentation, such as pre-eclampsia. |
| Starting Page | 1971 |
| Ending Page | 1971 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/molehr/10/4/10.1093/molehr/gah031/2/gah031.pdf?Expires=1491309332&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=TRsUkGudZKHtohRsYl-c0-4nKJpHC5kKhI6JnyOarX3qZlIsxp~l0zbRy~7KQACoHwpBVcCr8gRhpv6sfOh3Ab84QtNt9lDZPGTFPUhSR-b24J6F~1Y4FJGLPvH~B~Ac2TNphrQHEGKjPmP8BIl~vY6PgpwYye0DxVwP8C9-tvaAfh7Rh3FxIWWR1Ypx02IfBaARzNrK0~TpXXaywRIuF1~YvLAMwLyc6tRx7MaNv2xMHPr4Cl26XzJxf48emVbFLZ7psOed~Tt0gzIdZJxExdUmAJIBlZ5~ozZehXxHf15cy-pE34mIziEDG2KLeNqHJrTOoolswdHcxfGtqiabVA__ |
| PubMed reference number | 14996996v1 |
| Volume Number | 10 |
| Issue Number | 4 |
| Journal | Molecular human reproduction |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Eclampsia Endothelial Growth Factors Extracellular Matrix FGF10 protein, human FGF4 protein, human Fibroblast Growth Factor Gelatinases Matrix Metalloproteinase 9 Placenta Placentation Plasminogen Activator Pre-Eclampsia Stroma Structure of cytotrophoblast Urokinase Vascular Endothelial Growth Factor A alteplase trophoblast |
| Content Type | Text |
| Resource Type | Article |
