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Control of serotonergic neurons in rat brain by dopaminergic receptors outside the dorsal raphe nucleus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Martín-Ruiz, Raúl Ugedo, Luisa Honrubia, María Angeles Mengod, Guadalupe Artigas, Francesc Casañas |
| Copyright Year | 2001 |
| Abstract | We studied the control of dorsal raphe (DR) serotonergic neurons by dopaminergic transmission in rat brain using microdialysis and single unit extracellular recordings. Apomorphine (0.5-3.0 mg/kg s.c.) and quinpirole (0.5 mg/kg s.c.) increased serotonin (5-HT) output in the DR and (only apomorphine) in striatum. These effects were antagonized by 0.3 mg/kg s.c. SCH 23390 (in DR and striatum) and 1 mg/kg s.c. raclopride (in DR). 5-HT(1A) receptor blockade potentiated the 5-HT increase produced by apomorphine in the DR. Apomorphine (50-400 microg/kg i.v.) increased the firing rate of most 5-HT neurons, an effect prevented by SCH 23390 and raclopride. Quinpirole (40-160 microg/kg i.v.) also enhanced the firing rate of 5-HT neurons. When applied in the DR, neither drug increased the 5-HT output in the DR or striatum. Likewise, micropressure injection of quinpirole (0.2-8 pmol) failed to increase the firing rate of 5-HT neurons. In situ hybridization showed that the dopamine (DA) D(2) receptor transcript was almost absent in the DR and abundant in the substantia nigra (SN) and the periaqueductal grey matter (PAG). Using dual probe microdialysis, the application of tetrodotoxin or apomorphine in SN significantly increased the DR 5-HT output. Thus, the discrepancy between local and systemic effects of dopaminergic agonists and the absence of DA D(2) receptor transcript in 5-HT neurons suggest that DA D(2) receptors outside the DR control serotonergic activity. |
| Starting Page | 21S |
| Ending Page | 21S |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://digital.csic.es/bitstream/10261/33068/1/j.1471-4159.2001.00275.x.pdf |
| PubMed reference number | 11331405v1 |
| Volume Number | 77 |
| Issue Number | 3 |
| Journal | Journal of neurochemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apomorphine Cell Nucleus Dopamine Agonists Dopamine Hydrochloride Dopamine Receptor Dual Gray Matter Microdialysis Neostriatum Nucleic Acid Hybridization Quinpirole Raclopride SCH 23390 Serotonergic Neurons Serotonin Substantia nigra structure Tetrodotoxin Transcript dorsal raphe nucleus mg/kg picomole (pM) raphe nuclei |
| Content Type | Text |
| Resource Type | Article |
