NDLI: Determinação a baixo custo de açúcares redutores totais em caldo-de-cana, empregando sistemas de análise por injeção em fluxo com o uso de DNS

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Determinação a baixo custo de açúcares redutores totais em caldo-de-cana, empregando sistemas de análise por injeção em fluxo com o uso de DNS

Content Provider	Semantic Scholar
Author	Barros, Osmar Soares
Copyright Year	2005
Abstract	Low cost determination of total reducing sugars in sugar cane broth using a flow injection analysis system with DNS A flow injection analysis system was employed for determination of total reducing sugars in sugar cane broth. The method is based on hydrolysis of sucrose followed by oxidation of reducing sugars by 3,5-dinitrosalicylic acid (DNS) in alkaline solution and spectrophotometric determination at 510 nm. In order to obtain better sensitivity and selectivity, the parameters sample volume and reactor length were studied to evaluate the behavior of the analytical curves. Aiming the use of low reagents amount and a low cost in FIA system, fish aquarium mini-compressors were used instead of peristaltic pumps and acrylic spectrophotometric 0011 instead of imported glass cell. The present system was compared to Lane-Eynon method recommended by the Brazilian Ministry of Agriculture. Using the t-test no sígnificant differences were observed between both methods, with relative deviations around of 1%. The method allows analyses of ca. 14 samples hwith relative standard deviation lower than 1.35%.
File Format	PDF HTM / HTML
DOI	10.11606/D.46.2016.tde-05072016-130132
Alternate Webpage(s)	https://www.teses.usp.br/teses/disponiveis/46/46133/tde-05072016-130132/publico/Osmar_Soares_Barros_Mestrado.pdf
Alternate Webpage(s)	https://doi.org/10.11606/D.46.2016.tde-05072016-130132
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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