Methods for imaging thick specimens: confocal microscopy, deconvolution, and structured illumination.

Content Provider	Semantic Scholar
Author	Murray, John M.
Copyright Year	2011
Abstract	When a thick specimen is viewed through a conventional microscope, one sees the sum of a sharp image of an in-focus region plus blurred images of all of the out-of-focus regions. High background, scattering, and aberrations are all problems when viewing thick specimens. Several methods are available to deal with these problems in living samples. These methods can be grouped into three classes: primarily optical (e.g., confocal microscopy, multiphoton microscopy), primarily computational (e.g., deconvolution techniques), and mixed (e.g., structured illumination) approaches. This article describes these techniques, which make it possible to see details within thick specimens (e.g., the interiors of cells within living tissue) by optical sectioning, without the artifacts associated with physically sectioning the specimen.
File Format	PDF HTM / HTML
DOI	10.1101/pdb.top066936
PubMed reference number	22135661
Journal	Medline
Volume Number	2011
Issue Number	12
Alternate Webpage(s)	http://cshprotocols.cshlp.org/content/2011/12/pdb.top066936.full.pdf
Alternate Webpage(s)	https://doi.org/10.1101/pdb.top066936
Journal	Cold Spring Harbor protocols
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article