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Isolation of nuclei from Aspergillus nidulans protoplasts.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vágvölgyi, Csaba Ferenczy, Lajos |
| Copyright Year | 1991 |
| Abstract | Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological state, as demonstrated by fluorescence microscopy and electronmicroscopy. The weight ratio of DNA:RNA:protein was 1:3.0:10.8 in the nuclear fraction. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1574-6968.1991.tb04889.x |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsle/82/3/10.1111/j.1574-6968.1991.tb04889.x/2/82-3-247.pdf?Expires=1498029686&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=b5zQQPfzULjIIBLcKwZdJscuyIdewGNLfdwN2GDAEYp~L44PArr8b7yYjqtCpamJkq8hYo9vzMjfJ-tPhatItTB3Rv7HIRU-XwiNbYeHkf763ilMxrJvz39EccspkpY7U3vZwNDheG1PE-TS0kPjuiqtnclnRhvxKaouDXFfXFsYF3fq~KudmOB8MFRsdGNLAF77JWHXTTYefxeCAAg65j27cW~AUlCZkDu-gSxVQ4ZWbvfGu6TfJrXx3eHZDmoOoJ12A-pXFis2TP1c1fZduhLrWEiONFtzkSEGOqBtQEh-peMeb5OxzkWP2ZhNzeWyHC~PwMBEwqiQA-8tXmxQBg__ |
| PubMed reference number | 1769520 |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1574-6968.1991.tb04889.x |
| Journal | Medline |
| Volume Number | 66 |
| Issue Number | 3 |
| Journal | FEMS microbiology letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |